Cell Analysis & Imaging Lab: 18 Resources found

THIS RESERVATION IS FOR EQUIPMENT USE ONLY. SCREEN TAPES MUST BE RESERVED SEPARATELY. TapeStation reservations include sample preparation, instrument run time, data export, and cleanup. Please reserve enough time to complete the entire workflow. The TapeStation system combines an instrument, data processing software, reagents, and ScreenTape devices specifically designed for nucleic acid analysis. It processes samples automatically, requiring only 1-2 μL of sample volume. Analysis time is approximately 1-2 minutes per sample. This instrument is for the analysis of DNA and RNA samples. It is used to quantify, size, and assess the integrity of these biomolecules. The system runs automated electrophoresis, providing precise analytical evaluation. The 4200 TapeStation system can analyze up to 96 samples with fully automated processing in a single run. Minimum reservation time: 1 hour.

· The rate is charged per screen tape. Please order enough screen tapes to accommodate all of your samples. · 16 sample slots per screen tape: 1 ladder slot and up to 15 sample slots. · Size range 35-1000 bp · Quantitative Range 0.1-50 ng/ul

· The rate is charged per screen tape. Please order enough screen tapes to accommodate all of your samples. · 16 sample slots per screen tape: 1 ladder slot and up to 15 sample slots. · Size range 35-1000 bp · Quantitative Range 10-1000 pg/ul

· The rate is charged per screen tape. Please order enough screen tapes to accommodate all of your samples. · 16 sample slots per screen tape: 1 ladder slot and up to 15 sample slots. · Size range 100-6000 nt · Quantitative Range 500-10000 pg/ul · RIN Functional Range 1000-25000 pg/ul

· The rate is charged per screen tape. Please order enough screen tapes to accommodate all of your samples. · 16 sample slots per screen tape: 1 ladder slot and up to 15 sample slots. · Size range 100-6000 nt · Quantitative Range 25-500 ng/ul · RIN Functional Range 25-500 ng/ul

Bio-Rad CFX Connect Real-Time (Quantitative) PCR System (Do not reserve separately for NextGen) The CFX Connect system can be used for running and analyzing large gene expression studies. The system’s unsurpassed thermal performance plus its innovative optical design produce accurate, reliable data, and powerful, yet intuitive software accelerates every step of your real time PCR research. The optimal result could be collected using minimized amount of reagents and sample in very short period of time. CFX system can determine the concentration of generated DNA sample libraries prior to running them on the Experion 1K DNA chip assay and sequencing on the NextSeq 500. A qPCR validation/quantification will be required prior to sequencing for the accurate quantification of good quality libraries. The system is optimized for Bio-Rad factory calibrated dyes, but can incorporate reagents from different manufacturers. The CFX manager software allows for quick setup and analysis.

MINIMUM OF ONE HOUR RESERVATION The MA900 supports a wide range of applications. The system offers flexibility with a choice of 4 excitation lasers - 488 nm, 405 nm, 561 nm and 638 nm on two beam spots. Free-form PMTs enable detection of fluorescence signals from each beam spot, allowing detection of up to 12 fluorescence parameters and 2 scatter parameters. Microfluidics sorting chips are available in 3 sizes 70um, 100um and 130um for sorting into 2-way and 4-way tubes and 96 and 384 well plates.

Additional pricing for academic and government groups is available upon request.

Frozen tissue section prep. Self users provide their own slides, slide box, and OCT-embedded tissue blocks. An additional fee will apply to use the slides at the Cell Analysis and Imaging Lab.

The Amnis® FlowSight® Imaging Flow Cytometer has three excitation lasers (405, 488, 642nm) and twelve standard detection channels that simultaneously produce brightfield, darkfield or side scatter, and up to ten channels of fluorescence imagery of every event at ~20X magnification with high sensitivity CCD cameras. The FlowSight® provides users with the ability to gain detailed images of a large number of cells in a relatively short period of time and the opportunity to perform a range of novel applications including co-localization, internalization, stem cell differentiation, cell cycle, phagocytosis, and cell-cell interactions. The instrument configuration includes with fluorescence amplification and minimal noise detection that results in very high signal sensitivity paired with a powerful quantitative image (QI) processing option. Data files can be analyzed using Amnis® IDEAS® software for image processing alongside traditional gating strategies or saved in .fcs format for analysis through alternate software types (i.e. FlowJo or ModFit™). In addition to being able to see the cells displayed in dot plots and histograms, the imaging provides analysis tools not available in conventional cytometers such as texture and spot counting. Booking the Instrument: The time required for each sample is dependent on the cell concentration, the number of events needed and the complexity of the compensation strategy. A good general guideline for booking is 10 minutes per sample to start. Additionally, if your experiment requires a DNA dye or potential contaminants to subsequent experiments there will need to be a sterilization step (45min) which needs be included in the booking of the machine by the user. (This will be determined by CAI Staff when Data Acquisition Form is submitted and based on the experimental design.) We do not currently accept MRSA or BSL-2 samples.

Sample Characterization Equipment Laser Scanning Confocal Additional pricing for academic and government groups is available upon request.

Rapid quantification of DNA, RNA, or Protein.

The Spectral AMI-HT provides unrivaled sensitivity for bioluminescence and fluorescence in vivo imaging. AMI-HT acquires quantitative images from a diverse set of applications including well plates, plants and small animals. Designed around the needs of animal scientists, the Spectral AMI IVIS Imaging system include a robust build, patented LED illumination source, custom filter options, -90°C cooled camera, and absolute calibration to enable high throughput imaging. · 5 Mouse Capacity (BLI/FLI) - 25cm x 17cm field of view. · LED Fluorescence Illumination · 10 Wavelengths/10 Filters. Additional filters can be added/changed by operator, if needed · Mouse-friendly heated Platform allows you to easily recover wandering mice · Anesthesia compatible with all third party gas anesthesia systems · -90°C Cooled Camera. Ultra cold for maximum sensitivity · Absolute Calibration ensures quantifiable data http://spectralinvivo.com/imaging-systems/

Section tissue that has been fixed and embedded in either paraffin or resin. The microtome will enable to cut sections 0.5um to 100um in thickness. Self-users provide their own slides, slide box, and paraffin-embedded tissue blocks. An additional fee will apply to use the slides at Cell Analysis and Imaging Lab.

Tissue-Tek SEC 6 is a tissue embedding station which is used to embed tissue samples in paraffin wax. This station features a digital programmable interface which enables to program individual temperature settings for the paraffin reservoir, cassette bath, mold warmer and work surface.

The Tecan Safire is a versatile, multimode microplate reader designed for absorbance, fluorescence intensity, and luminescence measurements. It supports a wide range of life science and biochemical assays, making it well suited for academic research, method development, and teaching laboratories. - Multimode detection: absorbance, fluorescence, and luminescence - Monochromator-based optics for flexible wavelength selection - Compatible with 6- to 384-well microplates - Temperature control for kinetic and enzyme-based assays - Magellan Software - Common applications include enzyme kinetics, protein quantification, cell-based assays, and reporter gene studies

Fragment DNA to between 150 bp and 5000 bp. for downstream experiments such as molecular cloning or Next Generation Sequencing. Rate charge is in 15 minute increments (≤10 tubes per increment).