Analytical Chemistry Lab: 5 Resources found

ACL Technical Assistance


Industry Rates
Special:$261/hr
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Gel Permeation (GPC)
Waters Alliance 2695
Gel Permeation Chromatography (GPC) Located in Saab/ETIC 114 THF ONLY BASED SYSTEM Gel Permeation Chromatography (GPC) also referred to as Size Exclusion Chromatography (SEC) is a powerful and versatile analytical technique for understanding and predicting polymer performance. It is a mode of liquid chromatography which separates components of a mixture based on size. In GPC/SEC, larger molecules elute from the column first, and smaller molecules elute later. This technique is used for understanding and predicting polymer performance. It is the most convenient technique for characterizing the complete weight distribution of a polymer. GPC can determine several important parameters: o Number average molecular weight o Weight average molecular weight o Molecular weight distribution o polydisperity GPC is performed on a Waters Alliance 2695 HPLC system with PDA detector and refractive index detector. We use Waters Styragel HR columns 1,3, and 4 for molecular weight determination of up to 600K. This system is run using 100% tetrahydrofuran(THF only) as the mobile phase at a flow rate of 0.9ml/min. Each sample must be dissolved in 100% tetrahydrofuran for a few hours before analysis at 10 to 20 mg/ml, and filtered through a 0.45 micron nylon or PTFE filter. Polystyrene standards from a molecular weight of 500 to 400K are used to create the calibration curve. Each GPC run takes one hour to complete. There is a 105 sample carousel for automatic injections. Empower 2 is the software system for controlling the separation and analysis. Training takes approximately one to two hours. Please contact wendy_gavin@uml.edu for any questions, training or service
2 Documents Available


Industry Rates
Self:$71/hr
Service:$331/hr
Training:$331/hr
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High Performance Liquid Chromatography
Agilent 1100
High Performance Liquid Chromatography (HPLC) Located in Saab/ETIC 308 HPLC is one of the most powerful tools in analytical chemistry. It has the ability to separate, identify, and quantitate the compounds that are present in any sample that can be dissolved in a liquid. HPLC could be used to understand the elemental composition of a drug substance, chemical substance, or food product. The CRF offers a C18 Reverse Phase column with acetonitrile, methanol, and water as mobile phases. Triflouroacetic acid is offered as a mobile phase additive. Qualitative and Quantitative analysis is possible. We offer an Agilent 1100 HPLC system equipped with auto-sampler and PDA detector. Up to 5 different wavelengths can be analyzed at the same time. Chemstation software controls the HPLC experiments and data anaylsis. Training takes 2 to 4 hours depending on type of analysis, experience, and method development. We offer training to become self-users as well as service options. Please contact wendy_gavin@uml.edu for questions, training or service.
2 Documents Available


Industry Rates
Self:$71/hr
Service:$331/hr
Training:$331/hr
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High Performance Liquid Chromatography
Waters Alliance 2695
We offer a Waters Alliance 2695 HPLC system equipped with a Waters 2996 photodiode array detector, and Waters 410 refractive index detector. Empower 3 software is used to analyze sample data and purity. It contains a quaternary pump, auto sampler and column oven. Training takes 2 to 4 hours depending on type of analysis, experience, and method development. We offer training to become self-users as well as service options. Please contact wendy_gavin@uml.edu for questions, training or service.


Industry Rates
Self:$71/hr
Service:$331/hr
Training:$331/hr
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Liquid Chromatograph Mass Spectrometer (LC/MS)
Shimadzu 8040
Shimadzu LC/MS Triple Quadrupole Located in Saab/ETIC 308 The Shimadzu 8040 Triple Quadrupole LC/MS is an analytical system that combines the separating power of liquid chromatography with the qualitative and quantitative power of mass spectrometry. Mass spectrometry detectors offer information including molecular weight, structural information, purity, quantity, and identity of sample. The LC/MS can analyze samples from small molecule to large proteins and peptides. It can measure a m/z ratio of up to 2000. Ionization modes include electrospray interface (ESI) and dual ion source (DUIS). Duis enables ESI, APCI, positive and negative ionization in the same analysis, significantly improving efficiency by simultaneously analyzing a wide variety of compounds in a single run. Data Acquisition modes offered are: · SCAN · SIM-selected ion monitoring · Multiple Reaction Monitoring (MRM) · product ion scan · precursor ion scan · neutral loss scan modes. Scanning in positive and negative modes occurs simultaneously. Using LabSolutions software, the LC/MS offers automation, method optimization, and data analysis techniques. The CRF offers training to become independent users or services to run samples for our customers. We offer a C18 RP column along with acetonitrile, methanol, and water for mobile phases. Users are required to supply their own columns, HPLC methods, mobile phase additives, and a properly prepared sample. All mobile phases, columns, and samples must be approved for use in LC/MS before any analysis. Training requires 1 to 4 hours before self use. The scheduling of samples, training, and services must be approved by contacting Wendy Gavin at wendy_gavin@uml.edu prior to signing up for a reservation on the CRF website.
4 Documents Available


Industry Rates
Self:$111/hr
Service:$371/hr
Training:$371/hr