NGS & Genomics: 14 Resources found

BCL Technologist


Industry Rates
Service:$224/hr
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Bio-Rad CFX Connect Real-Time System
Bio-Rad CFX Connect Real-Time (Quantitative) PCR System (Do not reserve separately for NextGen) The CFX Connect system can be used for running and analyzing large gene expression studies. The system’s unsurpassed thermal performance plus its innovative optical design produce accurate, reliable data, and powerful, yet intuitive software accelerates every step of your real time PCR research. The optimal result could be collected using minimized amount of reagents and sample in very short period of time. CFX system can determine the concentration of generated DNA sample libraries prior to running them on the Experion 1K DNA chip assay and sequencing on the NextSeq 500. A qPCR validation/quantification will be required prior to sequencing for the accurate quantification of good quality libraries. The system is optimized for Bio-Rad factory calibrated dyes, but can incorporate reagents from different manufacturers. The CFX manager software allows for quick setup and analysis.


Industry Rates
Self:$70/hr
Service:$285/hr
Training:$285/hr
Bioanalyzer - Electrophoresis System
Agilent Technologies
This instrument needs to be reserved with the Bioanalyzer DNA, or RNA chip. This instruments is for the analysis of DNA, RNA, or protein. It is used to quantify and size these biomolecules. The system runs similarly to standard gel electrophoresis, however, the added advantage of this instrument is its ease of use, accurate sizing and quantitative ability. Note: This is a closed system so antibody detection, or probing is not possible following a run.


Industry Rates
Self:$70/hr
Service:$285/hr
Training:$285/hr
Bioanalyzer DNA Chip
See Bioanalyzer Instrument. The Bioanalyzer DNA chip should be reserved when running a DNA chip on the Bioanalyzer. Do not reserve for NGS


Industry Rates
Special:$56/ea
Bioanalyzer RNA Chip
See Bioanalyzer Instrument. The bioanalyzer chip should be reserved when running RNA on the Bioanalyzer. Do not reserve for NGS.


Industry Rates
Special:$32/ea
DNA/RNA Isolation
Isolation of DNA and/or RNA from simple (soft) tissues or cells using our standard DNA or RNA isolation procedure. If working with a hard-to-lyse sample or a sample that is complex in nature, please contact Jack Lepine at Jack_Lepine@uml.edu to make arrangements for this work. Downstream applications may include PCR, qPCR, molecular cloning, CRISPR, or DNA/RNA Sequencing. One hour includes up to 20 samples.


Industry Rates
Self:$127/hr
Service:$306/hr
Training:$306/hr
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Flow Cytometer
EMDMillipore
The Amnis® FlowSight® Imaging Flow Cytometer has three excitation lasers (405, 488, 642nm) and twelve standard detection channels that simultaneously produce brightfield, darkfield or side scatter, and up to ten channels of fluorescence imagery of every event at ~20X magnification with high sensitivity CCD cameras. The FlowSight® provides users with the ability to gain detailed images of a large number of cells in a relatively short period of time and the opportunity to perform a range of novel applications including co-localization, internalization, stem cell differentiation, cell cycle, phagocytosis, and cell-cell interactions. The instrument configuration includes with fluorescence amplification and minimal noise detection that results in very high signal sensitivity paired with a powerful quantitative image (QI) processing option. Data files can be analyzed using Amnis® IDEAS® software for image processing alongside traditional gating strategies or saved in .fcs format for analysis through alternate software types (i.e. FlowJo or ModFit™). In addition to being able to see the cells displayed in dot plots and histograms, the imaging provides analysis tools not available in conventional cytometers such as texture and spot counting. Booking the Instrument: The time required for each sample is dependent on the cell concentration, the number of events needed and the complexity of the compensation strategy. A good general guideline for booking is 10 minutes per sample to start. Additionally, if your experiment requires a DNA dye or potential contaminants to subsequent experiments there will need to be a sterilization step (45min) which needs be included in the booking of the machine by the user. (This will be determined by BCL Staff when Data Acquisition Form is submitted and based on the experimental design.) We do not currently accept MRSA or BSL-2 samples.


Industry Rates
Self:$101/hr
Service:$316/hr
Training:$316/hr
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Fluorometer (Qubit)
Thermo Fisher Scientific Qubit 3.0
Do not reserve this instrument separately for NextGen. Rapid quantitation of DNA, RNA, or Protein.


Industry Rates
Self:$70/hr
Service:$285/hr
Training:$285/hr
iSeq 100 Next Generation Sequencer
Illumina
The iSeq is a next generation sequencing device made by Illumina Inc. It is the smallest instrument in the fleet of Illumina sequencers capable of producing small data sets and is thus reduces the cost per sample compared to a NextSeq500 sequencer for bacterial genome/transcriptome sequencing, targeted sequencing, and viral genome sequencing. It is capable of producing 8M reads per run and can be run in read lengths of: -1 x 36bp -1 x 50bp -1 x 75bp -2 x 75bp -2 x 150bp


Industry Rates
1 x 36bp:$1,328/Run
1 x 50bp:$1,365/Run
1 x 75bp:$1,439/Run
2 x 75bp:$1,661/Run
2 x 150bp:$2,031/Run
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Next Gen Sequencer
Illumina NextSeq500


Industry Rates
Self:$101/hr
Service:$316/hr
Training:$316/hr
NGS-KIT
Reagents priced per sample. Select appropriate kit: NGS-Kits-DNA LT NGS-Kits-Exome LT NGS-Kits-mRNA LT NGS-Kits-smallRNA NGS-Kits-Total RNA LT


Industry Rates
DNA LT:$54/ea
Exome LT:$217/ea
mRNA LT:$87/ea
smRNA:$158/ea
TotRNALT:$184/ea
NGS-Library Prep & QC
Prices include Q/C and Chip for up to 11 samples.


Industry Rates
Self:$468/Run
Service:$3,799/Run
Training:$3,799/Run
NGS-Sequencer
Illumina NextSeq500
Sequencing Rates per run. All pricing includes kits and total number of sequencing hours required per run. This instrument is used after DNA or RNA have been converted to NGS libraries at the core. Sequencing menu NGS-High Output 300 cycles NGS-High Output 150 cycles NGS-High Output 75 cycles NGS-Mid Output 300 cycles NGS-High Output 150 cycles This instrument is used for sequencing DNA or RNA libraries to detect genomic variants in any organism. It can be used to detect single nucleotide polymorphisms (SNPs), insertions and deletions (INDELS), gene duplication events, and chromosomal rearrangements. Additionally, sequencing total RNA, small RNA, or mRNA can reveal information about gene expression levels, presence/absence of gene expression, gene splice variants, gene fusion events, and SNPs.


Industry Rates
HO300:$6,934/Run
HO150:$4,576/Run
HO75:$2,348/Run
MO300:$3,937/Run
MO150:$2,841/Run
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Ultra-Sonicator
Covaris M-220
Fragment DNA to between 150 bp and 5000 bp. for downstream experiments such as molecular cloning or Next Generation Sequencing.


Industry Rates
Self:$70/hr
Service:$285/hr
Training:$285/hr