Next Generation Sequencing & Genomics Lab: 13 Resources found

Bio-Rad CFX Connect Real-Time (Quantitative) PCR System (Do not reserve separately for NextGen) The CFX Connect system can be used for running and analyzing large gene expression studies. The system’s unsurpassed thermal performance plus its innovative optical design produce accurate, reliable data, and powerful, yet intuitive software accelerates every step of your real time PCR research. The optimal result could be collected using minimized amount of reagents and sample in very short period of time. CFX system can determine the concentration of generated DNA sample libraries prior to running them on the Experion 1K DNA chip assay and sequencing on the NextSeq 500. A qPCR validation/quantification will be required prior to sequencing for the accurate quantification of good quality libraries. The system is optimized for Bio-Rad factory calibrated dyes, but can incorporate reagents from different manufacturers. The CFX manager software allows for quick setup and analysis.

This instruments is for the analysis of DNA, RNA, or protein. It is used to quantify and size these biomolecules. The system runs similarly to standard gel electrophoresis, however, the added advantage of this instrument is its ease of use, accurate sizing and quantitative ability. Note: This is a closed system so antibody detection, or probing is not possible following a run. DNA chip is included in the hourly rate.

This instruments is for the analysis of DNA, RNA, or protein. It is used to quantify and size these biomolecules. The system runs similarly to standard gel electrophoresis, however, the added advantage of this instrument is its ease of use, accurate sizing and quantitative ability. Note: This is a closed system so antibody detection, or probing is not possible following a run. RNA chip is included in the hourly rate.

Please contact NGS Lab Manager, Jack Lepine (jack_lepine@uml.edu) for consultation prior to reserving this resource.

Do not reserve this instrument separately for NextGen. Rapid quantitation of DNA, RNA, or Protein.

The iSeq is a next generation sequencing device made by Illumina Inc. It is the smallest instrument in the fleet of Illumina sequencers capable of producing small data sets and is thus reduces the cost per sample compared to a NextSeq500 sequencer for bacterial genome/transcriptome sequencing, targeted sequencing, and viral genome sequencing. It is capable of producing 8M reads per run and can be run in read lengths of: -1 x 36bp -1 x 50bp -1 x 75bp -2 x 75bp -2 x 150bp

Please contact NGS Lab Manager, Jack Lepine (jack_lepine@uml.edu) for consultation prior to reserving this resource.

Reagents priced per sample. Select appropriate kit: NGS-Kits-DNA LT NGS-Kits-Exome LT NGS-Kits-mRNA LT NGS-Kits-smallRNA NGS-Kits-Total RNA LT

Prices include Q/C and Chip for up to 11 samples.

Sequencing Rates per run. All pricing includes kits and total number of sequencing hours required per run. This instrument is used after DNA or RNA have been converted to NGS libraries at the core. Sequencing menu NGS-High Output 300 cycles NGS-High Output 150 cycles NGS-High Output 75 cycles NGS-Mid Output 300 cycles NGS-High Output 150 cycles This instrument is used for sequencing DNA or RNA libraries to detect genomic variants in any organism. It can be used to detect single nucleotide polymorphisms (SNPs), insertions and deletions (INDELS), gene duplication events, and chromosomal rearrangements. Additionally, sequencing total RNA, small RNA, or mRNA can reveal information about gene expression levels, presence/absence of gene expression, gene splice variants, gene fusion events, and SNPs.

Fragment DNA to between 150 bp and 5000 bp. for downstream experiments such as molecular cloning or Next Generation Sequencing.