Cell Analysis & Imaging Lab: 8 Resources found

CAI Technical Assistance

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CELL SORTER Sony MA900.jpg
Cell Sorter
Sony MA900
MINIMUM OF ONE HOUR RESERVATION The MA900 supports a wide range of applications. The system offers flexibility with a choice of 4 excitation lasers - 488 nm, 405 nm, 561 nm and 638 nm on two beam spots. Free-form PMTs enable detection of fluorescence signals from each beam spot, allowing detection of up to 12 fluorescence parameters and 2 scatter parameters. Microfluidics sorting chips are available in 3 sizes 70um, 100um and 130um for sorting into 2-way and 4-way tubes and 96 and 384 well plates.

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Leica SP8 LSCM-CRF.jpg
Confocal Laser Scanning Microscope
Leica SP8
Additional pricing for academic and government groups is available upon request.

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Leica CM1850
Frozen tissue section prep. Self users provide their own slides, slide box, and OCT-embedded tissue blocks. An additional fee will apply to use the slides at the Cell Analysis and Imaging Lab.

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Flow Cytometer.jpg
Flow Cytometer
The Amnis® FlowSight® Imaging Flow Cytometer has three excitation lasers (405, 488, 642nm) and twelve standard detection channels that simultaneously produce brightfield, darkfield or side scatter, and up to ten channels of fluorescence imagery of every event at ~20X magnification with high sensitivity CCD cameras. The FlowSight® provides users with the ability to gain detailed images of a large number of cells in a relatively short period of time and the opportunity to perform a range of novel applications including co-localization, internalization, stem cell differentiation, cell cycle, phagocytosis, and cell-cell interactions. The instrument configuration includes with fluorescence amplification and minimal noise detection that results in very high signal sensitivity paired with a powerful quantitative image (QI) processing option. Data files can be analyzed using Amnis® IDEAS® software for image processing alongside traditional gating strategies or saved in .fcs format for analysis through alternate software types (i.e. FlowJo or ModFit™). In addition to being able to see the cells displayed in dot plots and histograms, the imaging provides analysis tools not available in conventional cytometers such as texture and spot counting. Booking the Instrument: The time required for each sample is dependent on the cell concentration, the number of events needed and the complexity of the compensation strategy. A good general guideline for booking is 10 minutes per sample to start. Additionally, if your experiment requires a DNA dye or potential contaminants to subsequent experiments there will need to be a sterilization step (45min) which needs be included in the booking of the machine by the user. (This will be determined by BCL Staff when Data Acquisition Form is submitted and based on the experimental design.) We do not currently accept MRSA or BSL-2 samples.

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Fluorescence Microscope
Olympus LSCM FV300
Sample Characterization Equipment Laser Scanning Confocal Additional pricing for academic and government groups is available upon request.
4 Documents Available

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Leica HistoCore Multicut
Section tissue that has been fixed and embedded in either paraffin or resin. The microtome will enable to cut sections 0.5um to 100um in thickness. Self-users provide their own slides, slide box, and paraffin-embedded tissue blocks. An additional fee will apply to use the slides at Cell Analysis and Imaging Lab.

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Paraffin Embedding Console
Sakura Tissue-Tek SEC 6
Tissue-Tek SEC 6 is a tissue embedding station which is used to embed tissue samples in paraffin wax. This station features a digital programmable interface which enables to program individual temperature settings for the paraffin reservoir, cassette bath, mold warmer and work surface.

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